BD FACSymphony™ A5 SE Cell Analyzer

Unlock the full potential of the BD FACSymphony™ platform with the flexibility of spectral unmixing or compensation workflows

Overview

The BD FACSymphony™ A5 SE Cell Analyzer offers the ability to:

Gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors
Unmix spectral data live in BD FACSDiva™ Software while retaining familiar compensation workflow
May improve resolution of difficult cells and populations using autofluorescence unmixing
Access spectral abilities on your BD FACSymphony™ A5 instrument with an A5 SE upgrade kit
Choose between spectral or compensation-based workflows, achieved through careful optical design and added software functionality

Learn more from the BD FACSymphony™ A5 SE Cell Analyzer brochure.

Features

The BD FACSymphony™ A5 SE Cell Analyzer offers an optical design to enable both spectral unmixing and compensation workflows

Algorithmically optimized filter set collects full spectrum of emitted light

Algorithm trained on available dyes to optimize placement of 48 detectors across five onboard lasers

Software functionality allows live spectral unmixing or compensation workflows in BD FACSDiva™ Software

Using the familiar BD FACSDiva™ Software workflow, assign a dye to a detector with the choice to analyze using compensation or spectral unmixing live during acquisition
Visualize spectral profile with spectral plots
Resolve populations with high autofluorescence using autofluorescence unmixing

Premium BD FACSymphony™ System technology enhances resolution

High-parameter cascade (HPC) arrays

Scalable detector array technology allows for up to 20 detectors on a single laser line
Enables square form fit and gallium arsenide (GaAs) PMTs for enhanced resolution

High-sensitivity GaAs PMTs

Reduce noise and increase sensitivity in red and near-infrared emission channels
Maximize resolution across visible spectrum by utilizing two or three GaAs PMTs off each laser line for emission of 720 nm or greater

Take your analysis to the next level with FlowJo™ v10.8 Software including improved BD FACSDiva™ Software support

Match spectral unmixing results between BD FACSDiva™ Software and FlowJo™ v10.8 Software so your visualizations are the same during acquisition or analysis
Use BD FACSDiva™ Software to unmix autofluorescence or unmix in FlowJo™ Software, which includes the AutoSpill and AutoSpread algorithms
FlowJo™ Software provides a number of useful tools for high-dimensional visualization and analysis of BD FACSymphony™ Cell Analyzer data including tSNE, OptSNE, UMAP, TriMap, EmbedSOM and more

Keep up to date on the latest plugins at FlowJo Exchange.

The BD^® High Throughput Sampler (HTS) and BD FACSFlow™ Supply System (FFSS) options are available for increased efficiency

The HTS option:

Automates and accelerates sample acquisition
Compatible with 96- and 384-well plates
<0.5% sample carryover in high-throughput mode

The FFSS option:

Increases capacity and ease of use while maintaining a stable fluidics pressure
Reduces daily maintenance by incorporating a 20-L BD FACSFlow™ Cubitainer

Performance

The BD FACSymphony™ A5 SE Cell Analyzer offers flexibility of spectral unmixing or compensation-based workflows in a single instrument

Gallium arsenide (GaAs) PMTs reduce noise and increase resolution in far red and near-infrared channels

What is spectral cytometry?

Spectral flow cytometry represents an alternative data acquisition and analysis strategy to conventional flow cytometry providing advantages in flexibility of fluorescent label inputs without having to change filters, as well as the ability to multiplex more fluorescent labels in one multicolor sample.

See our spectral workflow in BD FACSDiva™ Software

Get a sneak peek on how you can directly interact with image data in real time on a flow cytometer. Image and flow data on each event are integrated. You can easily view the image for an event by mousing over a dot on any scatter plot, even during acquisition. There is an image wall feature that allows you to look at images of all the events within a gate. While the sample is running, the image wall will update in real time to display the last 50 events that were analyzed.