Expand your T cell research with confidence
The BD Horizon™ Human T Cell Backbone Panel consists of an optimized configuration of core markers specifically designed to expand your research with recommended fluorochrome drop-ins and enable flexibility in panel design without compromising resolution.
Now you can focus more on incorporating additional markers to expand your panel and spend less time worrying about spillover and spread or its impact of new fluorochromes.
BD Horizon™ Human T Cell Backbone Panel Kit contains a set of markers that can be combined with up to five additional fluorochromes without impacting resolution
| Marker | Clone | Fluorochrome |
|---|---|---|
| CD3 | UCHT1 | BVS10 |
| CD4 | SK3 | BV786 |
| CD8 | RPA-T8 | R718 |
| CD45RA | HI100 | PE-Cy7 |
| CD197 (CCR7) | 2-L1-A | BV711 |
BD Horizon™ Brilliant Stain Buffer is included to provide optimal reagent performance
Example of recommended drop-in fluorochromes
| Laser | Filter set | Drop-in Fluoro-chrome |
|---|---|---|
| Violet 405 nm | BV421/V450/Pacific Blue™ | |
| Blue 488 nm | FITC/BB515/Alexa Fluor™ 488 | |
| Blue or Yellow-green 488 nm or 561 nm | RY586 | |
| Red 660 nm | APC/Alexa Fluor™ 647 | |
| UV 355 nm | BUV395 |
Drop-in reagents not included in backbone kit.
AF: Alexa Fluor™ Reagent
BB: BD Horizon Brilliant™ Blue Reagent, BUV: BD Horizon Brilliant™ Ultraviolet Reagent
BV: BD Horizon Brilliant™ Violet Reagent, R: BD Horizon™ Red Reagent
RY: BD Horizon RealYellow™ Reagent
The BD Horizon™ Human T Cell Backbone Panel Kit offers increased flexibility for more parameters
High Performance
- Strategically designed to be complemented with up to five drop-in markers with no resolution impact to the backbone or the selected drop-ins
- Verified protocols to ensure optimal performance
- Consistent performance across instruments with different optical configurations
Performance
Performance of backbone panel including drop-in configurations
Applications
Identification of FoxP3+ Treg subsets
A) The backbone panel was complemented with surface and intracellular markers conventionally used to identify human Treg subsets.
B) Clear resolution of all the subsets of interest was observed on fixed and permeabilized PMBCs from N = 3 healthy donors. Samples were acquired on a 4-laser BD FACSymphony™ A1 Cell Analyzer and analyzed with FlowJo™ Software v10.8.1.
B) Clear resolution of all the subsets of interest was observed on fixed and permeabilized PMBCs from N = 3 healthy donors. Samples were acquired on a 4-laser BD FACSymphony™ A1 Cell Analyzer and analyzed with FlowJo™ Software v10.8.1.
Upregulation of inhibitory receptors
RESOURCES
Development of a human T cell backbone flow cytometry panel enabling flexibility in reagent choice while minimizing panel design challenges