BD^® AbSeq Assay

Conduct advanced single-cell protein analyses alongside genomic assays in the same workflow

Overview

BD^® AbSeq Antibody-Oligonucleotide Conjugates together with the BD Rhapsody^TM mRNA Sequencing Kits enable simultaneous detection of proteins and mRNA expression in a single experiment and help deepen your understanding of protein biology and accelerate your single-cell research.

Check out our BD AbSeq Immune Discovery Panel

FEATURES

The BD^® AbSeq Assay provides enhanced cell type identification and high-dimensional protein profiling

The assay:

Elucidates complex biological systems with distinct clustering of different cell subsets
Enables interrogation of tens to hundreds of cell-surface protein markers in a single experiment
Integrates seamlessly with a catalog of RNA assays—whole transcriptome analyses, targeted RNA assays, TCR and BCR profiling and multiplexing—to serve as a single multiomics solution
Provides a broad range of specificities, all part of a validated BD antibody portfolio
Is designed for use with the BD Rhapsody™ Single-Cell Analysis Systems
Provides customer-friendly analyses through integration with the BD Rhapsody™ System’s bioinformatics pipeline
Eliminates the need to split rare or small samples for different assays by virtue of simultaneous mRNA and protein capture

APPLICATIONS

The BD^® AbSeq Assay enables simultaneous measurement of surface proteins and mRNA expression, facilitating the identification of distinct subsets of regulatory T cells

Detect up to 100 different cell surface markers using the BD^® AbSeq Assay with high confidence

The BD^® AbSeq Assay facilitates identification of specific changes in the myeloid cells of high-fat diet (HFD) mice.

Single-cell AbSeq and targeted mRNA-Seq analyses of myeloid cell populations localized in adipose tissue from control and HFD mice. t-SNE visualization of differences in expression of Adgre1 (encodes F4/80) gene expression and CD11c protein expression (identified via AbSeq) in different cell clusters of myeloid cells from the adipose tissue of HFD mouse.

The BD^® AbSeq Assay enables expression analysis of CD11c and CD5 markers in chronic lymphocytic leukemia (CLL) samples.

Multiomic analysis of CLL samples and healthy donors. t-SNE clustering of healthy and CLL patient samples using mRNA or surface protein only or a combination of both. Expression of key cell surface markers identified by the BD^® AbSeq Assay in both healthy and CLL samples.

Multiomic assay using BD^® AbSeq Conjugates provides more defined clustering of peripheral blood mononuclear cells

t-SNE clustering of PBMCs using mRNA or multiomic data. A, B. t-SNE coordinates are calculated for all PBMCs based on mRNA data only. Cells are colored based on cell type. C, D. Same coordinates as in A and B, but only T-cell subsets are displayed. Markers used for cell-type definition (protein data used for all markers except FcγRIIIa, for which mRNA data were used)—CD4 T-cells: CD3+CD4+; CD8 T-cells: CD3+CD8+; γδ T-cells: CD3+TCRγδ+; B cells: CD19+; Monocytes: CD14+; NK cells: CD3-CD45RA+ FcγRIIIA-high.