Single-Cell Multiomics Protocols

Sample Preparation

Sample Preparation and Cell Labeling

Preparing Single Cell Suspensions>

This protocol describes preparation of single cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.

Co-staining cells with fluorescent antibodies and BD Oligo-Conjugated Antibodies>

Here, we address how to co-stain cells with the same clone of both fluorescent antibodies and oligonucleotide-conjugated antibodies (BD® Single-Cell Multiplexing Kit or BD® AbSeq Ab-Oligos).

Single Cell Labelling with the BD Single-Cell Multiplexing Kit>

The BD Single-Cell Multiplexing Kit (Cat. No. 633781) utilizes an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the kit, referred to as a Sample Tag, has a unique sample barcode conjugated to a human universal antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system or other single cell analysis systems.

Single Cell Labelling with BD^™ AbSeq Ab-Oligos>

This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides).

dCODE Dextramer^® (RiO) staining protocol>

This protocol provides instructions on cell labeling with dCODE Dextramer® and optional BD AbSeq Ab-Oligos..

Single Cell Labelling with the BD Single-Cell Multiplexing Kit and BD^® AbSeq Ab-Oligos (1 plex to 40 plex)>

This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (1 plex to 40 plex) with the BD® Single-Cell Multiplexing Kit.

Single-Cell Labeling with BD Single-Cell Multiplexing Kit and BD^® AbSeq Ab-Oligos (41 plex to 100 plex)>

This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit.

BD^® AbSeq Immune Discovery Panel (IDP)>

The IDP consists of 30 different specificities against major human immune markers combined in a single tube. Designed and optimized to work on the BD Rhapsody™ Single-Cell Analysis System, the IDP is tested to work seamlessly with BD Rhapsody Single-Cell RNA Assays and Multiplexing Kits. In this brochure, we also describe steps for reconstituting the lyophilized IDP and staining cells

cDNA Synthesis

Single Cell Capture and cDNA Synthesis with the BD Rhapsody Express Single-Cell Analysis System>

This protocol describes preparation of single cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.

Co-staining cells with fluorescent antibodies and BD Oligo-Conjugated Antibodies>

Here, we address how to co-stain cells with the same clone of both fluorescent antibodies and oligonucleotide-conjugated antibodies (BD® Single-Cell Multiplexing Kit or BD^® AbSeq Ab-Oligos).

Single Cell Labelling with the BD Single-Cell Multiplexing Kit>

The BD Single-Cell Multiplexing Kit (Cat. No. 633781) utilizes an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the kit, referred to as a Sample Tag, has a unique sample barcode conjugated to a human universal antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system or other single cell analysis systems.

Single Cell Labelling with BD^™ AbSeq Ab-Oligos>

This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides).

dCODE Dextramer^® (RiO) staining protocol>

This protocol provides instructions on cell labeling with dCODE Dextramer® and optional BD AbSeq Ab-Oligos..

Single Cell Labelling with the BD Single-Cell Multiplexing Kit and BD^® AbSeq Ab-Oligos (1 plex to 40 plex)>

This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (1 plex to 40 plex) with the BD® Single-Cell Multiplexing Kit.

Single-Cell Labeling with BD Single-Cell Multiplexing Kit and BD^® AbSeq Ab-Oligos (41 plex to 100 plex)>

This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) (41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit.

BD^® AbSeq Immune Discovery Panel (IDP)>

The IDP consists of 30 different specificities against major human immune markers combined in a single tube. Designed and optimized to work on the BD Rhapsody™ Single-Cell Analysis System, the IDP is tested to work seamlessly with BD Rhapsody Single-Cell RNA Assays and Multiplexing Kits. In this brochure, we also describe steps for reconstituting the lyophilized IDP and staining cells.